Rules For Passing The Analysis Of Feces For Lamblia

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Rules For Passing The Analysis Of Feces For Lamblia
Rules For Passing The Analysis Of Feces For Lamblia

Video: Rules For Passing The Analysis Of Feces For Lamblia

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Video: Examination of stool, stool analysis - signs of disease © 2023, February
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Rules for passing the analysis of feces on lamblia

When observing the patient's symptoms such as feeling unwell, weakness, abdominal discomfort, atopic dermatitis, vomiting with elevated acetone, problems with appetite, pathology of the biliary system, doctors prescribe a feces or blood test to identify lamblia in the human body - the protozoa that provoke this a disease like giardiasis.

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The study of feces helps to identify lamblia in the human body.

How to take

The analysis is aimed at reliably determining the presence of lamblia cysts in the feces. This is the fastest and most accurate research method. He also diagnoses the form of the disease - chronic or acute.

It is necessary to conduct an examination immediately after detecting dangerous signs, because within a few days after infection, lamblia most easily detect their presence in the human body.

In the latter, you can order a comprehensive examination for the presence of parasites in the body or a number of diagnostic procedures designed to confirm or deny the presence of giardiasis in a patient.

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In the feces of a person infected with parasites, immobile cysts of lamblia can be found.

At the same time, motionless cysts of lamblia will be found in the feces of the infected, which are thus removed from the small intestine to the outside and represent a protective form of unicellular organisms.

Preparation for delivery

To get a truthful conclusion, you should carefully prepare for the analysis:

  1. Stop taking any antiparasitic and anthelmintic drugs a week before delivery.
  2. Limit the intake of other drugs, especially rectal ones, which can distort the clinical picture, if it is impossible, inform the doctor about them.
  3. Avoid taking antibiotics until results are obtained.
  4. 2-3 days before the delivery of feces, exclude too fatty foods from the diet, adhere to the basics of a healthy diet to the maximum.
  5. For 8-10 hours before bowel movements, refrain from eating, tea, coffee and juices.
  6. Avoid taking an analysis during the menstrual cycle in women or after an X-ray of the gastrointestinal tract.
A week before the delivery of feces for analysis, you should stop taking antiparasitic and anthelmintic drugs
A week before the delivery of feces for analysis, you should stop taking antiparasitic and anthelmintic drugs

One week before the delivery of feces for analysis, you should stop taking antiparasitic and anthelmintic drugs.

It is necessary to limit the intake of other medicines, especially rectal
It is necessary to limit the intake of other medicines, especially rectal

It is necessary to limit the intake of other medicines, especially rectal ones.

2-3 days before the delivery of feces, you should adhere to the maximum of the basics of a healthy diet
2-3 days before the delivery of feces, you should adhere to the maximum of the basics of a healthy diet

2-3 days before the delivery of feces, you should adhere to the basics of a healthy diet to the maximum.

8-10 hours before bowel movements, you need to refrain from eating
8-10 hours before bowel movements, you need to refrain from eating

8-10 hours before bowel movements, you need to refrain from eating.

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How to collect material

For the analysis of feces for lamblia, the material must be collected in a disposable container with a sealed lid (it is best to purchase it at a pharmacy) or in another sterile glass or plastic dry container.

It is not allowed to bring biomaterial in matchboxes or paper bags that absorb the liquid feces and thereby violate its consistency required for analysis.

The feces should be obtained after spontaneous defecation (without the help of enemas or suppositories, taking laxatives), and should not contain impurities.

Before defecation, you must first empty the bladder, so that during the collection of the analysis, urine does not mix with feces and does not provoke an error in the study.

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Before defecation, you need to empty the bladder so that during the collection process, urine does not mix with feces.

Before taking the analysis, you should thoroughly wash the anal area with a detergent (permissible - with a pharmaceutical solution of Furacilin) ​​and wipe off with a clean dry towel.

The presence of cysts in liquid feces is well detected, therefore, the posterior portion of the biomaterial should be preferred.

How to store

It is necessary to deliver the received analysis for examination as soon as possible.

In a hospital setting, feces should be examined within 20 minutes after delivery.

Fresh material should be delivered to the laboratory within 2 hours after its collection, since during this time the protozoa retain their mobility and their characteristic flagella, which facilitates the study.

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Fresh material should be delivered to the laboratory within 2 hours after collection.

In old feces, under the influence of the environment and cooling of the mass, cysts become hard, become covered with a thick membrane and lose flagella. These reasons make it difficult to diagnose them.

In case of impossibility of fast delivery, it is required to ask the laboratory where the analysis is made for special preservatives and use them to preserve the sample (according to the instructions received)

Manufacturing time

Analysis in the laboratory in the standard mode is done within 24 hours.

The employee conducts microscopy of the provided material, which is prepared using various reagents. The sample is applied to a glass slide and placed under a microscope.

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The sample is applied to a glass slide and placed under a microscope.

The initial smear intended for study is enriched and pre-stained with 1% Lugol's solution, since the cysts themselves are transparent.

If cysts of lamblia or adult organisms are found, then giardiasis is diagnosed.

Result

If the test is positive, the report will indicate the presence of Lamblia intestinalis cysts. Also, in the presence of infection, the sample will contain mucus, starch, leukocytes in the amount of 0-1 in the field of view.

Sometimes there are cases when decoding gives a false negative result, because traces of lamblia may not be presented in a single stool sample. Such a result can also be obtained if the rules for taking a sample are not followed or if there is poor preparation for the study.

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Sometimes the decoding gives a false negative result, because traces of lamblia may not be present in a single stool sample.

If, despite a negative result, there are all the corresponding symptoms, it is recommended to re-take the test after a few days. Experts believe that an accurate diagnosis of giardiasis is normally established after a threefold confirmed result.

Feces for antigen or PCR

The study of feces for antigen to lamblia is a more reliable research method that excludes the role of the human factor in diagnosis and is the best in its reliability.

The study reveals not the parasites themselves and their cysts, but antibodies produced by the immune system in response to the presence of pathogens in the body (monoclonal antibodies to the GSA-65 antigen). During the analysis, fragments, eggs and cysts of lamblia can be found. The peculiarity of the method is that it allows you to detect lamblia even in those periods when cysts and traces of eggs are not visible in fecal masses.

It is allowed to resort to research using PCR (polymerase chain reaction), detecting individual genes in the DNA of lamblia, but even in megacities this is a rare and expensive procedure.

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In the laboratory, the division and self-reproduction of the protozoan DNA is stimulated under the influence of reagents and temperature.

In the laboratory, the division and self-reproduction of the protozoan DNA is stimulated under the influence of reagents and temperature. Thanks to this, it is possible to achieve high accuracy (up to 98%).

Other diagnostic methods

If the analysis of feces has become uninformative or the reliability of its results is suspicious, a blood test for antibodies to lamblia (IgG, IgM, IgA) is prescribed. At the same time, doctors consider this method of research additional and it is impossible to diagnose giardiasis only on its basis.

Giardia antibodies in feces can be detected not only by coprogram or scraping, but also during:

  • immunohistochemical research (IHG);
  • enzyme immunoassay (ELISA).

Studies differ in the different method of enrichment of the samples taken before laboratory analysis.

How to get tested for children

Unlike adults, a feature of collecting biomaterial from infants is the need to free the baby from a disposable diaper in advance. Diapers or panties should be used. When collecting liquid feces in a sterile container, they should be taken from several places to increase the reliability of the result.

If the child is already using the pot, the item should be thoroughly washed with baby soap and rinsed with boiling water.

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