Analysis For Opisthorchiasis - How To Pass It Correctly

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Analysis For Opisthorchiasis - How To Pass It Correctly
Analysis For Opisthorchiasis - How To Pass It Correctly
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  • 1 Where and how is the delivery of feces?
  • 2 Features of the study of feces
  • 3 Test results and signs of disease

It is estimated that more than 20 million people are infected with a specialist opisthorchiasis, and most of them live in Russia. Different methods are used to detect the disease. One of the most effective is the analysis of feces for opisthorchiasis. Where and how the mass is surrendered for verification, what are the features of the study and the signs of the disease you will learn from the article.

Where and how is the delivery of feces?

how is the delivery of feces
how is the delivery of feces

If there is a suspicion of the development of opisthorchiasis in a person, then for an accurate diagnosis, the doctor recommends donating feces for opisthorchiasis and explain where to do it. The fact is that this disease is accompanied by a large variety of symptoms. Because of this, it is impossible to accurately determine the cause of such a reaction of the body. If the parasites are in the body for quite a long time, then the study of feces will help identify helminth eggs. Their number will determine the stage of the disease and suggest treatment methods.

Before you donate feces for analysis, it is necessary to carry out small preparatory measures. The first step is to empty the urine and carry out a hygienic cleaning of the external genital organs. These actions prevent urine from entering the feces, which increases the accuracy of the study.

In order to collect feces for the laboratory, it is recommended to use dry containers. You can use a duck-style container or stretch cling film over the toilet seat. Another requirement relates to the freshness of the stool. Therefore, most often the morning portion is rented, or everything is stored in the refrigerator.

To ensure the accuracy of the study, it is important to take into account other requirements on how to properly prepare for the delivery of feces:

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  • feces should be obtained without the use of laxatives;
  • if the patient is using drugs that stimulate intestinal motility, then therapy should be suspended for two days;
  • It is important to follow a healthy diet for several days.

Women are additionally required not to donate feces during menstruation. Otherwise, blood may enter the stool.

Features of the study of feces

analysis for the presence of parasites
analysis for the presence of parasites

Opisthorchias begin laying eggs only after a month of living in the patient's body. Eggs leave the body together with feces, in addition, they can be seen in the duodenal juice. In this regard, a comprehensive study is immediately carried out.

Usually an analysis is carried out for the presence of vital activity of parasites using a microscope. The feces are processed by special regents, and after that the search and counting of the number of eggs takes place. To obtain an accurate result, you will need to examine several stools. This is due to the fact that opisthorchis lay clutches in different places and irregularly.

In order to determine the number of eggs in a sample, one of the following methods is used:

  1. Deposition method Goryachev. The test feces are stirred in purified distilled water. Then add a 20% solution of potassium chloride, the resulting mixture is filtered through a metal sieve. After that, the particles of feces immediately begin to precipitate, while the eggs linger on the surface for some time. After three hours, the top layer is removed from the solution using a pipette. The remaining sediment is carefully examined under a microscope.
  2. Fulleborn's method. Feces delivered to the laboratory are processed in the same way as in the previous case. Only this method implies a longer settling of the material. After that, a thin part of the sediment is examined over a large area.
polymerase chain reaction
polymerase chain reaction

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A more modern version of laboratory analysis is the polymerase chain reaction. With its help, a fragment of the egg is taken and the decoding of foreign DNA is carried out. So you can determine the type of parasitic form of the organism and determine the most effective treatment.

Test results and signs of disease

analysis results
analysis results

The presence of parasites in the body is determined by direct examination of the samples. After mixing the feces with a certain volume of glycerin, the specialist performs flotation. After that, the counting of the number of eggs in the feces begins:

  • if there are up to 100 eggs in one gram of feces, then the infection is considered insignificant;
  • however, if the number exceeds 3000 units, then the disease is classified as severe.

In any case, even if only one egg was found, then we can definitely say about the presence of worms in the body. Still, there are times when it is difficult to establish a definitive diagnosis. So, a blood test may indicate infection, but there are no cysts in the feces at all. This could be due to three reasons:

  • a mistake was made during the study;
  • parasites have recently entered the body and have not yet begun to multiply;
  • too few pests.

If the analysis is doubtful, and the symptoms of the disease persist, re-sampling is carried out. Only after an accurate diagnosis has been made, the doctor prescribes active treatment. Otherwise, taking medications will only harm the patient. That is why you should not self-medicate and completely trust traditional medicine without consulting a doctor.

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