Trichomonas Culture Tank: Detection Of A Virus In A Smear

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Trichomonas Culture Tank: Detection Of A Virus In A Smear
Trichomonas Culture Tank: Detection Of A Virus In A Smear

Video: Trichomonas Culture Tank: Detection Of A Virus In A Smear

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Video: Trichomonas vaginalis(Life cycle, Lab diagnosis, Infection, Transmission)#trichomonasvaginalis 2023, February
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Tank sowing for Trichomonas. If Trichomonas is found in a smear, the patient is diagnosed with trichomoniasis or trichomoniasis. This inflammatory disease of the genitourinary system occurs not only in women, but also in men. Among all sexually transmitted diseases, trichomoniasis is especially widespread, so you should find out the causes of this pathology and eliminate all risk factors. Trichomoniasis is a very common sexually transmitted disease. Tank culture for Trichomonas is one of the modern methods of diagnosing the disease.

The content of the article:

  • 1 Trichomoniasis (Trichomonas)
  • 2 Symptoms of trichomoniasis
  • 3 Diagnosis of trichomoniasis in women
  • 4 Tests for trichomoniasis
  • 5 General information about the study

    • 5.1 What the research is used for
    • 5.2 When a study is scheduled
  • 6 Tank sowing for Trichomonas
  • 7 Treatment of trichomoniasis

Trichomoniasis (Trichomonas)

Trichomonas culture tank: detection of a virus in a smear
Trichomonas culture tank: detection of a virus in a smear

Trichomoniasis or trichomoniasis is an inflammatory disease of the genitourinary tract in men and women. It is caused by the simplest T.vaginalis (translated as Trichomonas vaginalis). Trichomonas is not a microbe, but a "unicellular animal", that is, a creature more adapted to antimicrobial drugs and living conditions in the human host organism.

Very often, trichomoniasis is mistakenly called gonorrhea, but it is not. Tripper is gonorrhea, another urogenital inflammatory disease caused by gonococcus. Trichomoniasis is sexually transmitted - it is not only traditional contacts, but also oral and anal.

The incubation period is 3-5 days (from the moment of infection until the first signs of disease appear), but given our era of antibiotics and antimicrobials, and their unreasonable prescription, I had to consult patients when trichomoniasis lasted up to 3 weeks.

Trichomoniasis symptoms

The first symptoms of the disease are foamy-mucous discharge in men and women, cramps when urinating, itching in the urethra, vagina. Quite often, when other microorganisms are attached, that is, concomitant infection, pus may also appear. But I already wrote above that trichomoniasis is a uniquely insidious, little-studied disease.

Once in the body, this parasite begins to secrete substances that suppress the normal human immune response.

Trichomanada in the human body becomes "own". According to the statistics of my patients (up to 80%), the disease is asymptomatic, and people deal with various types of complications when the chronic form is already "raging". According to the authors, the acute form lasts 2 months, but this is very conditional. It is shortened with re-infection, decreased immunity.

Diagnosis of trichomoniasis in women

Tank sowing for Trichomonas. During the first examination in the mirrors, the doctor takes a so-called general smear for bacterioscopic examination. For analysis for trichomoniasis, discharge from the posterior fornix of the vagina is taken. It is better if this analysis is done immediately - the beating of the cilia of Trichomonas and the high mobility of oval microorganisms are clearly visible under a microscope, and the result of the study is ready in 20 minutes.

This type of diagnosis allows you to establish the total number of microbes and the severity of the inflammatory response.

If the smear is examined after drying and staining, then the result is most often negative, because Trichomonas, when dried, loses its mobility and specific anatomy. In this case, diagnostic errors are possible, since the cells of the vaginal epithelium can be mistaken for Trichomonas.

Tank sowing for Trichomonas
Tank sowing for Trichomonas

For confirmation, sowing on flora is used - a culture method. Its meaning is that the discharge taken from the vagina is placed ("sown") on a special nutrient medium favorable for the reproduction of Trichomonas. Culture allows for the amount of pathogen and sensitivity to antibacterial drugs, which helps in prescribing the optimal treatment for trichomoniasis. Therefore, seeding can be used not only directly for diagnosis, but also in order to adjust the treatment method.

The most accurate method at the moment is considered the polymerase chain reaction for the determination of Trichomonas DNA (PCR). The accuracy of this study is about 95%, and the analysis is done in 1-2 days.

In doubtful cases, when diagnosing trichomoniasis, the direct immunofluorescence (DIF) method can additionally be used to detect antibodies to the infectious agent in the patient's blood.

Tests for trichomoniasis

Tank sowing for Trichomonas. If you notice the above symptoms and symptoms of complications, which I will talk about below, "take" your sexual partner and go to the doctor. I believe that initially it is a dermatovenerologist. Why?

1) Neither the gynecologist nor the urologist will take (if he is a "Specialist") to treat your other half, and this is important, the problem is common. Very often, treatment is prescribed to a partner without examining him, which is extremely unacceptable.

2) Only a dermatovenerologist can treat a couple, advise when you can start having sex after examination and treatment. If people are treated in different rooms, then we often have to deal with such cases: one sexual partner is cured, and the other is not, one doctor says that you can have a sex life, and the other continues examinations and treatment. This is how a person is made to follow the path of least "resistance …."

Tank sowing for Trichomonas
Tank sowing for Trichomonas

Sometimes the doctor, when a mini-surgical intervention is needed (for example, cauterization of cervical erosion) or examination (urethroscopy-instrumental examination of the urethra), may prescribe a consultation with a gynecologist or urologist.

I have come across people who explain the symptoms of trichomoniasis by saying that “I caught a cold” (or “caught a cold”), start taking completely unnecessary antibiotics, “drowning out” the infection, complicating the life of himself and the doctor, who will then inevitably treat you.

What tests will you have to take? Definitely - these are strokes. Men from the urethra, women from the vagina and urethra. It is best if the stronger sex comes to the doctor with morning urinary retention (to urinate in the evening, and not to urinate in the morning).

A blood test for trichomoniasis is ineffective and ineffective. Sometimes, when the doctor suspects you have an infection, he will ask for an injection (provocation) or advise you to drink beer or eat something spicy on the eve of taking smears. The fact is that Trichomonas feed on your iron, which is "taken" from erythrocytes (red blood cells). Therefore, the "cunning parasite" crawls deep into your tissues and "does its job."

In this case, it can be very difficult to find it. We often have to deal with patients who are dealing with complications of trichomoniasis and who have elevated white blood cells in the smear (cells responsible for the protective function of the body), but Trichomonas were not detected in any smear. Here the good old provocation comes to the rescue … It consists in sharply reducing the patient's immunity and creating favorable conditions for Trichomonas in order to identify them in the analyzes.

Tests for suspected urogenital infection, including trichomoniasis:

  • general blood analysis
  • general urine analysis
  • bacterioscopic analysis of secretions (smear)
  • PCR diagnostics
  • culture with determination of sensitivity to antibiotics and antifungal drugs

The main method for diagnosing trichomoniasis is the assessment by a gynecologist of the symptoms of trichomoniasis during a gynecological examination and the result of a vaginal smear analysis.

Tank sowing for Trichomonas
Tank sowing for Trichomonas

The identification of opportunistic flora accompanying trichomoniasis is helped by sowing vaginal discharge for dysbiosis with the simultaneous determination of the sensitivity of the flora to antibiotics. For a general assessment of the disease, it is also recommended to conduct studies on chlamydia, mycoplasma, HIV, hepatitis and other sexually transmitted infections, which are often detected in trichomoniasis.

The most informative are the analyzes of vaginal discharge - PCR diagnostics of trichomoniasis. PCR is a time-tested method of molecular diagnostics of STIs, including trichomoniasis. The PCR method allows you to determine the presence of the causative agent of trichomoniasis, even if only a few DNA molecules of the microorganism are present in the sample. The accuracy of PCR analysis, if performed correctly, reaches 100%. The PCR method for diagnosing STDs can detect even single cells of bacteria or viruses. PCR diagnostics detects the presence of the causative agent of trichomoniasis in cases where this cannot be done with the help of other tests.

PCR analysis is especially effective for diagnosing hidden forms of microorganisms, including ureaplasmas, which are often encountered in asymptomatic and chronic STDs.

With the help of PCR analysis, the causative agent of trichomoniasis is determined, and not the reaction to its introduction from the body. Thus, trichomoniasis can be diagnosed even in the incubation period and with its latent course, when there are no clinical or laboratory symptoms of trichomoniasis.

But even PCR diagnosis of trichomoniasis is not an ideal analysis. This is a very high-tech research method that requires compliance with the strictest rules of laboratory equipment. A sample of material for any analysis must be taken by a qualified physician, strictly following the instructions given by the laboratory. The PCR analysis results should be evaluated by the doctor who treats the particular patient.

Not always a positive PCR test result means the presence of trichomoniasis. False positive test results are possible if:

  • Tank sowing for Trichomonas
    Tank sowing for Trichomonas

    Not always a positive PCR test result means the presence of trichomoniasis

  • after a course of treatment for ureaplasmosis, the dead and no longer dangerous causative agent of infection is removed from the body by the immune system

Tank sowing for Trichomonas. False negative test results are possible even in the presence of obvious symptoms of urogenital infection. Possible reasons for this analysis result:

  • material for analysis was taken or investigated unsuccessfully
  • the woman took antibiotics less than a month before the test, or used suppositories, douching
  • it was not taken into account that before sampling material from the urethra, one should not urinate for 1 hour
  • the absence of the genome of the microorganism in the material sample (in the secretions) with the localization of the inflammatory process in the deep parts of the reproductive system (ovaries, fallopian tubes, prostate gland)

PCR analysis is a powerful and effective diagnostic tool that allows you to quickly and accurately find the causative agents of many STDs. Most often, it is used to diagnose specifically sexually transmitted infections. In any case, one cannot be limited to only one analysis. It is best to combine different tests and pass them several times with questionable results.

In addition to determining the infectious agent itself by PCR diagnostics, it is necessary to assess the body's immune response, which is determined by serological methods, for example, ELISA.

General information about the study

Tank sowing for Trichomonas. Urogenital trichomoniasis (trichomoniasis) is a widespread infectious disease of the genitourinary system, transmitted mainly through sexual contact. Its causative agent is the simplest Trichomonas vaginalis. The most commonly affected urethra and vagina (in women). The source of infection is a patient with trichomoniasis. The main route of transmission is sexual.

The incubation period for women is 5-28 days. Symptoms of the disease often appear (or intensify) with the onset of menstruation: foamy yellowish discharge from the vagina, itching and burning in it and in the vulva, pain during urination and during intercourse. The infection is often asymptomatic. During pregnancy, trichomoniasis can cause low birth weight or premature birth.

In men, trichomoniasis is accompanied by persistent white discharge from the urethra, but often there are no symptoms. Rarely, trichomoniasis causes balanoposthitis, prostatitis, epididymitis, cystitis.

The "gold standard" diagnosis of trichomoniasis is cultivation on nutrient media (inoculation). This is the most sensitive study. It is used, as a rule, to confirm the diagnosis or in cases where the results of microscopy are negative for symptoms of trichomoniasis.

What the research is used for

  • To establish the cause of inflammatory diseases of the genitourinary system.
  • To identify trichomoniasis or to confirm the diagnosis.
  • For differential diagnosis (along with other studies) of diseases occurring with similar symptoms, such as gonorrhea, chlamydia, mycoplasma, ureaplasma infection.

When the study is scheduled

  • With symptoms of trichomoniasis and negative microscopy results.
  • For chronic inflammatory diseases of the genitourinary system.
  • With positive serological test results.
  • When planning a pregnancy.
  • If the patient is known to have promiscuous intercourse.

Tank sowing for Trichomonas

Tank sowing for Trichomonas
Tank sowing for Trichomonas

Trichomoniasis is a disease of the genitourinary system caused by the simplest unicellular parasite Trichomonas vaginalis, better known as Trichomonas. The disease belongs to STDs, rare cases indicate the likelihood of infection through common household items or when visiting public baths, tanning salons, etc. One of the most effective methods for diagnosing trichomoniasis in our time is the Trichomonas culture tank. Bacterial culture, carried out in laboratory conditions, involves the collection of biological material from the patient, which is then placed in a special nutrient medium. The further course of the research is to cultivate and study Trichomonas for sensitivity to antibiotics of various groups.

Trichomoniasis in 75% of cases of the disease is accompanied by a diverse bacterial flora, while up to 40% of such patients are simultaneously infected with mycoplasmosis. If you do not do bacterial culture for Trichomonas, most of these patients will be classified in the group of bacterial urethritis.

In addition, cases were repeatedly recorded when, even after effective treatment, urogenital trichomonas of round shapes appeared in the patient's body. In some specimens, up to 3-5 nuclei were isolated, and the absence of flagella and undulating membrane was noted.

Scientists consider parasites of this form as passing through one of the stages of self-preservation of these protozoa. It has been proven that their appearance can lead to a relapse of the disease, but it is unlikely that it will be possible to diagnose them only with the help of smear microscopy, without making a culture tank for trichomoniasis.

In addition to the possibility of Trichomonas self-preservation in the body of a patient who underwent a course of treatment, the possibility of re-infection is not excluded. For this reason, patients who have a regular sexual partner are strongly advised to undergo treatment with him. Treatment options can only be selected by a doctor and only after receiving the results of all the necessary tests.

Trichomoniasis treatment

Tank sowing for Trichomonas
Tank sowing for Trichomonas

Tank sowing for Trichomonas. The main group of drugs in the treatment of trichomoniasis is 5-nitromedazoles (trichopolum, metrogil, tiberal..). It should be noted that the doses of the drug indicated on the package are small and ineffective. For the treatment of concomitant infection, broad-spectrum antibiotics are used (unidox, azitrox, etc.). In the chronic form, immunomodulators (pyrogenal, immunal….), Absorbable drugs (Lidaza, trypsin, wobenzym), drugs that improve the

intestinal flora (hilak-forte, normase…) are added. Local treatment in the form of baths and infusions into the urethra (chlorhexidine, miramistin ….).

Sexual life and drinking during treatment are strictly prohibited. It is advisable after treatment, especially the chronic form of trichomoniasis, to undergo a course of treatment with drugs that improve the intestinal microflora. A person is considered cured of trichomoniasis if Trichomonas is not found in women - within 2 monthly cycles, in men 2 weeks after treatment and also after a month.

Find out more:

  • Trichomoniasis symptoms: causes, signs and treatment
  • Trichomoniasis in women: symptoms, routes of transmission, treatment
  • Trichomoniasis - symptoms, causes, diagnosis and treatment

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