Stool Analysis - How Much Can You Store And When To Take

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Stool Analysis - How Much Can You Store And When To Take
Stool Analysis - How Much Can You Store And When To Take

Video: Stool Analysis - How Much Can You Store And When To Take

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Video: Comprehensive Stool Analysis 2023, February
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Stool analysis is taken by both children and adults. With the help of the analysis, you can determine the presence or absence of various diseases: helminthiasis, diseases of the gastrointestinal tract, liver, etc. If you want to get a reliable result when taking tests, then you must certainly find out how much you can store feces for analysis.

The content of the article:

  • 1 General rules for collecting stool analysis
  • 2 Types of stool tests

    • 2.1 Analysis of feces for dysbiosis
    • 2.2 Coprogram (general analysis of feces)
    • 2.3 Analysis of feces (scraping) for enterobiasis
    • 2.4 Analysis of feces for eggs of helminths (eggs of worms)
    • 2.5 Analysis of feces for occult blood
  • 3 General analysis of feces: how much feces can be stored in the refrigerator

    • 3.1 Storing stool in the coprogram refrigerator
    • 3.2 Storing stool in the refrigerator for analysis for egg worms
    • 3.3 Storing stool in the refrigerator for analysis for dysbiosis
  • 4 Cal for carbohydrates. How to assemble and store properly
  • 5 Analysis of feces for Helicobacter pylori. How to assemble and store properly
  • 6 Collection and storage of feces from children
  • 7 Features of collection and storage of stool analysis

General rules for collecting feces

In order for the fecal analyzes to be correct, you need to follow the general guidelines for collecting feces.

How long can stool analysis be stored
How long can stool analysis be stored

Recommendations for collecting feces:

  • feces for research are collected in a special sterile container with a screw cap and a spoon, which must be obtained in advance;
  • 3-4 days before the study, it is necessary to stop taking laxatives, castor and petroleum jelly, stop the administration of rectal suppositories and other drugs that affect digestion. Feces obtained after an enema, as well as after taking barium (during an X-ray examination) are not used for research;
  • women are advised to empty their bladder before collecting feces and pre-close the vagina with a cotton swab;
  • feces are collected after spontaneous defecation in a bedpan. The bed vessel must be clean, it is recommended to rinse it with running water several times and rinse it with boiling water. Then, with a spoon, feces are collected from 3-4 places of a single portion, placed in a disposable plastic container in an amount of no more than 1/3 of the container volume;
  • you should avoid getting impurities in the stool (urine, discharge from the genitals).

Types of stool tests

Analysis of feces for dysbiosis

Analysis of feces for dysbiosis: study of the human intestinal microflora. The analysis evaluates the concentration and ratio of "useful" (lactobacilli, bifidobacteria, E. coli), opportunistic (enterobacteria, staphylococcus, clostridia, fungi) and pathogenic (shigella, salmonella.) Microorganisms.

Due to various reasons, "beneficial" bacteria can disappear from the intestinal microflora and fungi of the genus Candida, Staphylococcus, Proteus, Pseudomonas aeruginosa can appear. 3-4 days before the study, you need to stop taking laxatives and stop the administration of rectal suppositories.

The amount of stool needed for analysis is about 10 ml. Feces are delivered to the laboratory within 3 hours from the date of collection of the analysis. It is advisable to keep the container with feces cold, but not freeze. This test will not be accepted in laboratories on Saturday and Sunday.

Feces obtained after an enema, as well as after an X-ray examination with barium, are unsuitable for analysis. If the patient is taking antibiotics, the analysis is done no earlier than 12 hours after drug withdrawal.

In what cases is an analysis of feces for dysbiosis prescribed:

  • unstable stools (constipation, diarrhea);
  • pain and discomfort in the abdomen, flatulence;
  • intolerance to a number of products;
  • skin rashes; · Allergic reactions;
  • intestinal infections; long-term therapy with hormones and anti-inflammatory drugs; determination of the nature of the violation of the normal intestinal biocenosis.

Also, the analysis is prescribed for newborns from the risk group and adolescents with frequent respiratory infections and allergic reactions.

Coprogram (general analysis of feces)

Coprogram (stool analysis): examination of the physical, chemical and microscopic characteristics of stool. The study of feces allows you to diagnose dysfunction of the stomach, pancreas, liver, the presence of accelerated passage of food through the stomach and intestines, malabsorption in the duodenum and small intestine; inflammatory process in the gastrointestinal tract, ulcerative, allergic, spastic colitis.

7-10 days before the test, cancel medications (all laxatives, bismuth, iron preparations, fat-based rectal suppositories, enzymes and other drugs that affect the processes of digestion and absorption). You can not do enemas the day before. After an X-ray examination of the stomach and intestines, the analysis of feces is possible no earlier than two days later.

The container with feces must be delivered to the laboratory on the day of collection of the material, and stored in the refrigerator (4-6 С0) before shipment. The analysis is prepared in 6 working days.

For 4-5 days, you must adhere to the following diet: milk, dairy products, cereals, mashed potatoes, white bread with butter, 1-2 soft-boiled eggs, some fresh fruit. Stool is collected after spontaneous bowel movement in a disposable plastic container with a sealed lid. Mixing urine with feces should be avoided.

Indications for the purpose of the analysis:

  • diagnosis of diseases of the digestive system;
  • evaluation of the results of the treatment.

Stool analysis (scraping) for enterobiasis

How long can stool analysis be stored
How long can stool analysis be stored

Analysis of feces (scraping) for enterobiasis: detection of pinworm eggs (helminths, the clinical manifestations of which are itching in the anal area and intestinal disorders). The study of feces for enterobiasis reveals the presence or absence of pinworm eggs.

In the morning, before defecation and holding the toilet of the genitals with a cotton swab dipped in glycerin, make a scraping from the surface of the folds around the anus. Place the stick in a plastic tube and close the lid tightly. Stool analysis is prepared in 1 working day. Normally, pinworm eggs are not found in the feces (scraping).

Indications for prescribing an analysis for enterobiasis:

  • suspected infection with pinworms (Enterobius vermicularis);
  • "Barrier" analysis (during hospitalization, registration of a medical record, a certificate to the pool, etc.)

Analysis of feces for eggs of helminths (eggs of worms)

Stool analysis for helminth eggs (worm eggs): detection of helminth eggs (worms), which cause a group of diseases called helminthiases. Most often in humans, the disease is caused by round and flat worms. The study of feces allows you to identify helminthiasis - ascariasis, trichinosis, hookworm infection.

Before being sent to the laboratory, the material should be stored in a refrigerator at 4 - 80 C. The analysis is prepared for 6 working days. Normally, eggs of worms are not found in feces.

The feces are collected in a disposable container with a screw cap and a spoon in an amount not exceeding 1/3 of the container volume. The material should be delivered to the laboratory on the same day. During collection, avoid urine impurities, genital secretions.

Indications for prescribing an analysis for helminth eggs:

  • suspicion of infection with helminths;
  • "Barrier" analysis (during hospitalization, registration of a medical book, etc.)

Fecal occult blood test

Analysis of feces for occult blood: - detection of "latent" blood, invisible during microscopic examination. Analysis of feces for occult blood shows the presence of bleeding from any part of the digestive tract.

For 7-10 days before the test, cancel medications (all laxatives, bismuth, iron preparations). You can not do enemas the day before. After an X-ray examination of the stomach and intestines, a fecal analysis is prescribed no earlier than two days later.

The container with feces must be delivered to the laboratory on the day of collection of the material, and stored in the refrigerator (4-6 С0) before shipment. The analysis is prepared in 6 working days. Feces for occult blood: negative. An indication for the appointment of an analysis is a suspicion of bleeding from any part of the gastrointestinal tract.

Before the analysis, exclude meat, liver and all foods containing iron (apples, bell peppers, spinach, white beans, green onions) from the diet three days in advance. Stool is collected after spontaneous bowel movement in a disposable plastic container with a sealed lid. Mixing urine with feces should be avoided.

General analysis of feces: how much feces can be stored in the refrigerator

Storing stool in the refrigerator for coprogram

The shelf life of fresh stool for the coprogram does not exceed eight hours, provided it is stored on the middle shelf of the refrigerator in a tightly closed container. The middle shelf of the refrigerator provides a temperature regime of 4–8 ° С.

Subzero temperatures in the first case or frequent temperature changes as a result of opening and closing the refrigerator in the second negatively affect the natural parameters of feces, which will distort the analysis result.

Under no circumstances should the container with biomaterial be placed in the freezer or on the side shelf of the refrigerator.

It is quite possible to store material on the middle shelf of the refrigerator from evening to morning. The main thing is that the stool storage time at room temperature is minimal, ideally it should not exceed 15-20 minutes.

Storing stool in the refrigerator for analysis for worm eggs

How long can stool analysis be stored
How long can stool analysis be stored

To study feces for helminth eggs, the material must be as fresh as possible. This is due to the fact that during long-term storage, the digestive enzymes contained in the feces cause lysis (dissolution) of the shell of the eggs of parasites, as a result, a false negative result is obtained.

In this case, special preservatives are used to increase the storage time of the material. The container with them should be obtained in advance from the laboratory where the analysis will be carried out. When using a preservative, feces on the worm eggs can be stored in the refrigerator for 4–6 hours.

Storing stool in the refrigerator for analysis for dysbiosis

Feces for analysis for dysbiosis must be delivered to the laboratory no later than three hours after collection. During this time, it is advisable to store the container at a temperature of 4-8 ° C, overlaying it with ice cubes or placing it in a refrigeration bag.

In extreme cases, stool can be stored in the refrigerator for up to 5-6 hours, however, the reliability of the result is considered reduced.

Mandatory conditions for collecting and storing feces for analysis for dysbiosis:

  • prevention of freezing of feces;
  • preventing the storage of the collected material before sowing on nutrient media for more than 6 hours;
  • use for collection of material only obtained in the laboratory or purchased from a pharmacy a sterile single-use container;
  • storage and transportation of feces to the laboratory only in a tightly closed container.

Feces for carbohydrates. How to assemble and store properly

One of the specific types of analyzes is the analysis of feces for carbohydrates. In the overwhelming majority of cases, it is assigned to babies in the first year of life. Feces for carbohydrates of newborns can reveal the so-called lactase deficiency.

In other words, to determine if a child has an intolerance to milk and derivatives, which often happens at a young age. Indications for the analysis of feces for carbohydrates can be symptoms such as unstable stools, increased gas production, accompanied by pain, and bloating.

The material you need can be collected by yourself using a special spoon and a sterile container. 5-10 ml is sufficient. The container must be delivered for subsequent analysis of feces for carbohydrates during the same day. It is best to keep the container tightly closed in the refrigerator until transport.

If the baby cries before going to the toilet for a big, or right after, you should pay increased attention to this. Perhaps you are at risk. Adult patients are also prescribed to have a stool test for carbohydrates. Chronic pancreatitis, for example, can cause carbohydrates to not be absorbed.

Remaining not split, they move down into the intestines, causing fermentation, irritable bowel syndrome, causing a lot of anxiety. Whether this is so, helps to find out the analysis of feces for carbohydrates. Regular loose stools, without taking laxatives and not provoked by any diet, is also a reason for examination.

How long can stool analysis be stored
How long can stool analysis be stored

You don't have to do anything special. If the analysis is to be passed to the baby, then you need to try to spend some time without a diaper. Feces must be collected from a clean oilcloth, but in no case from a tissue or cellulose surface.

In children and adults, the indicators of the norm are different. In a baby of the first year of life, a carbohydrate content exceeding 1% is a cause for serious concern, while for adult patients this figure alone does not mean anything. The laboratory assistant also evaluates the physical characteristics of the stool, for example, signs of fermentation, as well as odor. The results of the study are recorded, and the final diagnosis is always made by the doctor.

Analysis of feces for Helicobacter pylori. How to assemble and store properly

The analysis of feces for Helicobacter pylori is qualitative, that is, it answers the question of whether Helicobacter pylori is present in the body or not. The study is carried out in the laboratory by the method of polymerase chain reaction (PCR). The analysis is highly accurate - up to 95%. This analysis is the simplest type of diagnosis. It is recommended for children, the elderly and seriously ill patients.

Also, you can not take drugs that stimulate gastric motility. The feces are collected in a special container purchased from a pharmacy. If the container is 1/3 full, this will be enough for analysis.

How long can stool analysis be stored
How long can stool analysis be stored

The patient should not take any antibiotics for at least one month before the study. In addition, three days before the sampling of the biomaterial, it is necessary to abandon the "coloring" products (beets, grapes, black currants, red wine and others) and products containing coarse fiber (cabbage, carrots, radishes, radishes, beets, bran).

It is necessary to deliver the material to the laboratory for analysis as soon as possible. The maximum storage time is 10–12 hours at a temperature of + 2… -8 degrees Celsius.

In no case should you take feces from the toilet - disinfectants can get into the biomaterial. This analysis takes one day to prepare. Since the research is qualitative, the form may contain one of two answers: “negative” or “positive”.

Collection and storage of feces in children

With the advent of a child, mothers have to learn a lot. Including learning to take tests for a child. One of these tests is stool analysis. Having received a referral for stool analysis, some mothers do not know what to do next.

How long can stool analysis be stored
How long can stool analysis be stored

For almost all analyzes, feces are collected in the same way (with minor clarifications for some analyzes). For the child, feces are collected from a pot, from a diaper or diaper, immediately after the act of defecation. Adults from a carefully sanitized vessel or toilet.

For the analysis, you need a small amount of feces -1-2 teaspoons. It is best to take your morning feces for analysis. But if this is not possible. You can collect stool in the evening and store it in a tightly sealed jar or container for no longer than twelve hours on the bottom shelf in the refrigerator.

Feces are collected in a dry, clean glass jar with a lid or in a special container from a pharmacy. If you bought a container for stool analysis from a pharmacy, there is a special plastic spoon for collecting material.

Features of collection and storage of stool analysis

For some types of stool tests, you must adhere to certain rules for collecting and storing stool.

How long can stool analysis be stored
How long can stool analysis be stored

Features of collection and storage of feces:

in the case of X-ray examination of the stomach and intestines:

the study of feces for scatology is shown not earlier than two days after the procedures. It is advisable to use the following diet: milk, dairy products, cereals, mashed potatoes, white bread with butter, 1-2 soft-boiled eggs, some fresh fruit. Such food is taken within 4-5 days, feces are examined for 3-5 days (subject to self-emptying of the intestines);

  • feces for occult blood. (immunochromatographic method): Do not collect during or within 3 days after the menstrual period, and don’t donate feces in case of hemorrhoidal bleeding or blood in the urine. Alcohol, aspirin, or other drugs taken in excess can irritate the gastrointestinal tract, leading to latent bleeding. You must stop taking these drugs at least 48 hours before testing. There is no need to introduce any other dietary restrictions before detecting occult blood in feces by the immunochromatographic method;
  • when examining feces for parasites: it is advisable to take feces three times with an interval of 3-4 days. To control the treatment, send all the portions of feces allocated on the days of taking the anthelmintic agent.

Stool samples should be delivered to the laboratory no later than 8-10 hours after a bowel movement. For bacteriological studies, feces should be delivered to the laboratory as soon as possible. Stores should be stored until analysis and transported at + 4 ° C … + 8 ° C.

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